Journal: Frontiers in Immunology

Article Title: Exploring the role of mitochondrial metabolism and immune infiltration in myocardial infarction: novel insights from bioinformatics and experimental validation

doi: 10.3389/fimmu.2025.1543584

Figure Lengend Snippet: Experimental Validation of Hub MitoDEGs Expression and Immune Cell Markers. (a) Immunostaining of protein expression of COX5B, NDUFA2, NDUFS6 and UQCR11; (b) Western blotting assay of COX5B, NDUFA2, NDUFS6, UQCR11, BCL-2 and BAX (n=3, *p < 0.05); (c) Immunofluorescence Staining for Cleaved caspase-3; (d) Immunofluorescence Staining for F4/80, CD163 and CD86.

Article Snippet: Endogenous peroxidase activity was blocked using methanol for 15 min. After a 1 h treatment with 5% bovine serum albumin, the sections were sealed and incubated overnight with primary antibodies, including COX5B (dilution 1:200, Abclonal, Inc.), NDUFA2 (dilution 1:200, Abclonal, Inc.), NDUFS6 (dilution 1:200, Proteintech Group, Inc.), and UQCR11 (dilution 1:200, Proteintech Group, Inc.).

Techniques: Biomarker Discovery, Expressing, Immunostaining, Western Blot, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: High Concentrations of Atmospheric Ammonia Induce Alterations in the Hepatic Proteome of Broilers ( Gallus gallus ): An iTRAQ-Based Quantitative Proteomic Analysis

doi: 10.1371/journal.pone.0123596

Figure Lengend Snippet: List of differentially expressed nutrients metabolic proteins in hepatic samples from treatment group and control group.

Article Snippet: D0VX28 , Cytochrome b-c1 complex subunit 6 OS = Gallus gallus PE = 1 SV = 1 - [D0VX28_CHICK] , UQCRH , 0.31 , 0.0290 , Mitochondrial electron transport.

Techniques: Control, Activity Assay, Binding Assay, Modification, Histone Deacetylase Assay, Ubiquitin Proteomics, Protein Binding, Marker